Biological Safety Cabinet Services
NATA testing and regular maintenance of all Biological Safety Cabinets, Laminar flow cabinets and Clean workstations is critical to ensure safety and sterility of products. AES Environmental provides a comprehensive NATA testing and certification services for all clean air applications. Regular NATA testing of all cabinets should be completed at least once a year to ensure longevity and functionality of the cabinet.
AES is a NATA accredited ILAC IEC/ISO 17025 accredited organisation that provides specialist services for Biological or Microbiological Safety Cabinets, including:
Mechanical Inspection: This includes the motor/ blower unit, vibration mountings, switch functions, gaskets, pre-filters and HEPA filters.
Check for System Integrity: Challenge for filter leakage to AS 1807:2021 Cl 4.4 and AS 1807.7
Integrity of work zone: Test for induced air leakage in the work zone.
Prevent cross contamination in the work area: check and adjust air velocity and uniformity of airflows.
Establish HEPA filter life and performance: measure static pressure of filters and electronic motor current.
Check environment factors: measure light output, sound level and germicidal ultraviolet lamp efficiency.
Repair and adjust to specification: when necessary and whenever possible, on-site repairs are included in our services.
Risk Group 1Low individual and community riskBSC not required (unless significant aerosol) | Risk Group 2Moderate individual risk, limited community riskSuitable in Class I or Class II BSC |
Risk Group 3High individual risk, limited to moderate community riskSuitable in Class I or Class II BSC | Risk Group 4High individual and community riskSuitable only in class III BSC |
A Biological safety cabinet is an enclosed, ventilated workspace, and the various types are identified by the degree of biocontainment or operator protection required. Biological safety cabinets are available in three classes, to suit a wide range of applications and risk factors with products used within the depending on the protection required for the sample, operator or environment.. this includes:
Class I : Suitable for Operator protection only.
Class II: Suitable for Operator and product protection
Class III: Suitable for product and environment protection
The most common type of Biological Safety Cabinet is the Class II Type A2, by definition this is a room recirculating unit consisting of a laminar HEPA, Exhaust HEPA and typically twin-fans. The BSC will have 70% of it’s air recirculating through the laminar HEPA and 30% will be discharged through the exhaust HEPA filter.
Biological safety cabinets are required to be tested and certified at least annually or if there is any change to the cabinets function and/ or the position. Depending on the cabinet, the requirements for testing changes. See Table 1 to show the testing requirements according to AS2252. 1 (Biological safety cabinet class I) and AS 2252.2 (Biological safety cabinet class II).
AS 1807:2021 Testing Requirements | Class I | Class II | Class III* |
---|---|---|---|
AS 1807:2021 – Cl 4.1 Determination of air velocity and uniformity | n/a | ✓ | n/a |
AS 1807:2021 – Cl 4.2 Determination of fan blower performance under loaded filter conditions | ✓** | ✓** | ✓** |
AS 1807:2021 – Cl 4.3 Determination of work zone integrity | n/a | ✓ | ✓ |
AS 1807:2021 – Cl 4.4 Determination of integrity of terminally mounted HEPA | ✓ | ✓ | ✓ |
AS 1807:2021 – Cl 4.5 Determination of illuminance | ✓ | ✓ | ✓ |
AS 1807:2021 – Cl 4.6 Determination of vibration | ✓ | ✓ | ✓ |
AS 1807:2021 – Cl 4.7 Determination of sound level | ✓ | ✓ | ✓ |
AS 1807:2021 – Cl 4.8 Determination of inward air velocity of class I safety cabinet | ✓ | n/a | n/a |
AS 1807:2021 – Cl 4.9 Determination of air barrier containment | n/a | ✓ | n/a |
AS 1807:2021 – Cl 4.10 Determination of UV intensity | ✓ | ✓ | ✓ |
AS 1807:2021 – Cl 4.11 Determination of gas tightness | n/a | ✓ | ✓ |
AS 1807:2021 – 4.12 Determination of air barrier containment | n/a | ✓ | n/a |
Class I Biological Safety Cabinets are constructed for protection for the operator. In the cabinet, the air is drawn across the workspace into a HEPA filter, which prevents any harmful contamination escaping into the work zone opening.
Class II are open fronted biological cabinets, which provide protection from hazardous biological agents for personnel and the environment and also protect materials used within the cabinet.
Class II cabinets are generally preferable for uninoculated cell culture work or similar operations in which protection of the work is paramount.
Class III cabinets are enclosed work spaces where a physical barrier exists between the work zone and the operator. The operator will manipulate or process materials in the work zone through glove ports. The air within the cabinet is filtered on entry and exhaust to provide both a sterile work zone and prevent release of hazardous particulate.
Decontamination is essential before annual re-certification or major repairs when A biological safety cabinet comes into contact with risk groups 2, 3 or 4. Once the decontamination process has taken place, verification of decontamination will take place using biological indicators. Additionally, the decontamination and verification process should also take place prior to filter changes.
AES employs the latest technology for decontamination of class II BSC’s and can provide validated reports in compliance with AS 2467 and the current Biosecurity Containment Requirements.